Direct Observation of t-Butyl Alcohol Frozen and Sublimated Samples Using Low-Vacuum Scanning Electron Microscopy.
نویسندگان
چکیده
منابع مشابه
Direct observation of t-butyl alcohol frozen and sublimated samples using low-vacuum scanning electron microscopy.
Frozen biological specimens in t-butyl alcohol were examined under a low-vacuum environment in a "wet SEM" or "variable pressure SEM (scanning electron microscope)" equipped with a cooling stage and highly sensitive backscattered detector of the YAG type. After fixation with glutaraldehyde and osmium tetroxide, rat tissue blocks (tracheae and kidneys), and cultured human carcinoma cells were de...
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ژورنال
عنوان ژورنال: Archives of Histology and Cytology
سال: 1998
ISSN: 0914-9465,1349-1717
DOI: 10.1679/aohc.61.93